PURIFICATION OF MACROPHAGE-MIGRATION INHIBITORY FACTOR (MIF) FROM BOVINE BRAIN CYTOSOL

被引：41

作者：

GALAT, A

RIVIERE, S

BOUET, F

机构：

[1] Département d'Ingénierie et d'Etudes des Protéines (DIEP), Centre d'Etudes de Saclay (CEN-Saclay), F-91191 Gif-sur-Yvette Cedex

来源：

FEBS LETTERS | 1993年 / 319卷 / 03期

关键词：

MACROPHAGE MIGRATION INHIBITORY FACTOR; CIRCULAR DICHROISM; SECONDARY STRUCTURE PREDICTION;

D O I：

10.1016/0014-5793(93)80553-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two isoforms of the macrophage migration inhibitory factor (MIF) have been isolated to homogeneity from bovine brain cytosol. In agreement with the cDNA sequence of their human counterpart, they both have an apparent molecular weight of 12 kDa and are characterized by the following N-terminal amino acid sequence NH2-PMFVVNTNVPRASVPDGLLSELTQQLAQATGKPPQYIAV-. CD spectra revealed that bovine MIF contains 42% (+/- 3%) alpha-helix and 21% (+/- 3%) beta-structure. CD-constrained prediction of the secondary structure assigned MIF to the alpha/beta-class of proteins.

引用

页码：233 / 236

页数：4

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