DIRECT DETECTION OF EPSTEIN-BARR-VIRUS DNA FROM A SINGLE REED-STERNBERG CELL OF HODGKINS-DISEASE BY POLYMERASE CHAIN-REACTION

被引：21

作者：

TERAMOTO, N ^{[1
]}

AKAGI, T ^{[1
]}

YOSHINO, T ^{[1
]}

TAKAHASHI, K ^{[1
]}

JEON, HJ ^{[1
]}

机构：

[1] OKAYAMA UNIV,SCH MED,DEPT PATHOL 2,2-5-1 SHIKATA CHO,OKAYAMA 700,JAPAN

来源：

JAPANESE JOURNAL OF CANCER RESEARCH | 1992年 / 83卷 / 04期

关键词：

PCR; EPSTEIN-BARR VIRUS; HODGKINS DISEASE; MICROMANIPULATOR;

D O I：

10.1111/j.1349-7006.1992.tb00110.x

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Eleven cases of Hodgkin's disease (HD) were examined for the presence of the Epstein-Barr virus (EBV) genome, using the polymerase chain reaction (PCR) to detect EBV DNA in whole paraffin-embedded tissue specimens and in single cells picked out from the specimens with a micromanipulator. The EBV genome was detected in 5 of the 11 cases by conventional PCR. Single cell PCR demonstrated the EBV genome in Reed-Sternberg cells from all the EBV-positive cases, but not from any of the EBV-negative cases. Background lymphocytes and lysozyme-positive histiocytes from EBV-positive cases did not contain the EBV genome. These results indicate an etiological association of EBV with some cases of HD.

引用

页码：329 / 333

页数：5

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