RAPID AND EFFECTIVE WESTERN BLOTTING OF HISTONES FROM ACID-UREA-TRITON AND SODIUM DOCECYL SULFATE POLYACRYLAMIDE GELS - 2 DIFFERENT APPROACHES DEPENDING ON THE SUBSEQUENT QUALITATIVE OR QUANTITATIVE-ANALYSIS

An improved method for the electrophoretic transfer of histones from sodium dodecyl sulfate (SDS) and acetic acid-urea-Triton X-100 (AUT) polyacrylamide gels onto nitrocellulose membranes is described. In the case of SDS-gels, it was not essential to equilibrate them before transfer while for the AUT-gels, an equilibration step is essential to prevent the interference of Triton X-100 with the binding of histones to nitrocellulose. Transfer efficiency was different for different histone classes. Two procedures were developed: (i) one suitable for qualitative studies, and (ii) another for quantitative transfer.