POTENT PROTECTIVE EFFECT OF MELATONIN ON IN-VIVO PARAQUAT-INDUCED OXIDATIVE DAMAGE IN RATS

被引：181

作者：

MELCHIORRI, D ^{[1
]}

REITER, RJ ^{[1
]}

ATTIA, AM ^{[1
]}

HARA, M ^{[1
]}

BURGOS, A ^{[1
]}

NISTICO, G ^{[1
]}

机构：

[1] UNIV ROMA TOR VERGATA, DEPT BIOL, I-00173 ROME, ITALY

来源：

LIFE SCIENCES | 1994年 / 56卷 / 02期

关键词：

MELATONIN; PARAQUAT; LIPID PEROXIDATION; LUNG; LIVER;

D O I：

10.1016/0024-3205(94)00928-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The in vivo effect of melatonin on paraquat-induced oxidative damage in rat lung and liver was studied using two parameters: the concentration of malonaldehyde and 4-hydroxyalkenals as indices of lipid peroxidation; changes in total and oxidized glutathione. Melatonin (10mg/kg) or an equal volume of saline were administered intraperitoneally (ip) to rats 30 min prior to an ip injection of paraquat (20mg/kg or 70mg/kg). After paraquat treatment, the animals received melatonin or saline ip injections every six hours for 24 hours. Rats were killed 24 hours after paraquat injection. In lung, both the low and high dose of paraquat, when administered with saline, augmented lipid peroxidation (100% and 18%, respectively) above levels found in control animals. Treatment with melatonin completely reversed this effect. In liver, paraquat (70 mg/kg) increased lipid peroxidation by 40% over the levels of control animals. The increase was completely abolished by treatment with melatonin. Paraquat at 20mg/kg did not induce any significant change in liver lipid peroxidation. Paraquat treatment resulted in a significant decrease of total glutathione concentration and increased oxidized glutathione in both lung and liver. These effects were abolished by treatment with melatonin. The results suggest that melatonin confers marked protection against paraquat-induced oxidative toxicity in both the lung and liver.

引用

页码：83 / 89

页数：7

共 26 条

[1] REDUCTION OF PARAQUAT TOXICITY BY SUPEROXIDE-DISMUTASE [J].

AUTOR, AP .

LIFE SCIENCES, 1974, 14 (07) :1309-1319

[2] ACCIDENTAL POISONING BY PARAQUAT - REPORT OF 2 CASES IN MAN [J].

BULLIVANT, CM .

BMJ-BRITISH MEDICAL JOURNAL, 1966, 1 (5498) :1272-+

[3] PARAQUAT - MODEL FOR OXIDANT-INITIATED TOXICITY [J].

BUS, JS ;

GIBSON, JE .

ENVIRONMENTAL HEALTH PERSPECTIVES, 1984, 55 (APR) :37-46

[4]

ESTERBAUER H, 1990, METHOD ENZYMOL, V186, P407

[5] BIPYRIDYLIUM QUATERNARY-SALTS AND RELATED COMPOUNDS .5. PULSE-RADIOLYSIS STUDIES OF REACTION OF PARAQUAT RADICAL WITH OXYGEN - IMPLICATIONS FOR MODE OF ACTION OF BIPYRIDYL HERBICIDES [J].

FARRINGTON, JA ;

EBERT, M ;

LAND, EJ ;

FLETCHER, K .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 314 (03) :372-381

[6]

FISHER HK, 1973, AM REV RESPIR DIS, V107, P246

[7]

Girotti A W, 1985, J Free Radic Biol Med, V1, P87

[8]

GRIFFITH O.W., 1985, METHOD ENZYMAT AN, P521

[9]

Hardeland R., 1993, CHRONOBIOLOGY CHRONO, P113

[10] THE ROLE OF XANTHINE-OXIDASE IN PARAQUAT INTOXICATION [J].

KITAZAWA, Y ;

MATSUBARA, M ;

TAKEYAMA, N ;

TANAKA, T .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 288 (01) :220-224

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