CHARACTERIZATION OF THE 70KDA COMPONENT OF THE HUMAN KU AUTOANTIGEN EXPRESSED IN INSECT CELL-NUCLEI USING A RECOMBINANT BACULOVIRUS VECTOR

被引：34

作者：

ALLAWAY, GP ^{[1
]}

VIVINO, AA ^{[1
]}

KOHN, LD ^{[1
]}

NOTKINS, AL ^{[1
]}

PRABHAKAR, BS ^{[1
]}

机构：

[1] NIDDKD,BIOCHEM & METAB LAB,BETHESDA,MD 20892

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 168卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)92385-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Ku autoantigen is a human nuclear, DNA-binding heterodimer of 70kDa and 86kDa proteins. It is the target of autoantibodies in several autoimmune diseases. We now report the expression of a cDNA encoding the 70kDa Ku protein. Large amounts of protein were obtained using a recombinant baculovirus vector, in contrast with earlier unsuccessful attempts using other expression systems. We demonstrate that the 70kDa Ku protein is targeted to the nucleus and is associated with the nuclear matrix when expressed in the absence of the 86kDa Ku component. No post-translational modifications were observed. The 70kDa protein binds double and single-stranded DNA with very high affinity. Our results suggest that the baculovirus expression system may be of widespread use in the production and characterization of human autoantigens. © 1990.

引用

页码：747 / 755

页数：9

共 27 条

[1] LOCALIZATION OF HUMAN HISTONE GENE TRANSCRIPTS PREDOMINANTLY IN THE NONMATRIX NUCLEAR FRACTION [J].