TRANSFORMATION OF THE FILAMENTOUS FUNGUS GIBBERELLA-FUJIKUROI USING THE ASPERGILLUS-NIGER-NIAD GENE ENCODING NITRATE REDUCTASE

被引：25

作者：

SANCHEZFERNANDEZ, R

UNKLES, SE

CAMPBELL, EI

MACRO, JA

CERDAOLMEDO, E

KINGHORN, JR

机构：

[1] UNIV ST ANDREWS, PLANT MOLEC GENET UNIT, SIR HAROLD MITCHELL BLDG, ST ANDREWS KY16 9TH, FIFE, SCOTLAND

[2] UNIV SEVILLA, DEPT GENET & BIOTECN, E-41080 SEVILLE, SPAIN

来源：

MOLECULAR AND GENERAL GENETICS | 1991年 / 225卷 / 02期

关键词：

GIBBERELLA-FUJIKUROI; HETEROLOGOUS TRANSFORMATION; NITRATE REDUCTASE;

D O I：

10.1007/BF00269853

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per mu-g DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.

引用

页码：231 / 233

页数：3

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