DIRECT ELECTROCHEMICAL MEASUREMENT OF NITRIC-OXIDE RELEASED FROM HUMAN PLATELETS

被引：180

作者：

MALINSKI, T ^{[1
]}

RADOMSKI, MW ^{[1
]}

TAHA, Z ^{[1
]}

MONCADA, S ^{[1
]}

机构：

[1] WELLCOME RES LABS,RES LABS,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 194卷 / 02期

关键词：

D O I：

10.1006/bbrc.1993.1914

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A porphyrinic microsensor has been used to investigate the release of nitric oxide (NO) from human platelets in whole blood and in washed platelet suspensions. Basal release of NO was not detectable. Aggregation of platelets by collagen (1-15 μg/ml) but not by thrombin (0.1 U/ml) resulted in a concentration-dependent release of NO. This release was prolonged and potentiated by L-arginine (100-1000 μM) and inhibited by NG-monomethyl-L-arginine (300 μM). These data support our previous findings that human platelets generate NO during aggregation. © 1993 Academic Press, Inc.

引用

页码：960 / 965

页数：6

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