AVIAN CATHEPSIN-B CDNA - SEQUENCE AND DEMONSTRATION THAT MESSENGER-RNAS OF 2 SIZES ARE PRODUCED IN CELL-TYPES PRODUCING LARGE QUANTITIES OF THE ENZYME

被引：17

作者：

DONG, SS

STRANSKY, GI

WHITAKER, CH

JORDAN, SE

SCHLESINGER, PH

EDWARDS, JC

BLAIR, HC

机构：

[1] UNIV ALABAMA, DEPT PATHOL, BIRMINGHAM, AL 35294 USA

[2] DEPT VET AFFAIRS MED CTR, BIRMINGHAM, AL 35294 USA

[3] WASHINGTON UNIV, SCH MED, DEPT PHYSIOL & CELL BIOL, ST LOUIS, MO 63110 USA

[4] WASHINGTON UNIV, JEWISH HOSP ST LOUIS, SCH MED, DEPT MED, DIV RENAL, ST LOUIS, MO 63110 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1995年 / 1251卷 / 01期

基金：

美国国家卫生研究院;

关键词：

OSTEOCLAST; CATHEPSIN B; THIOL PROTEINASE;

D O I：

10.1016/0167-4838(95)00103-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Overlapping cDNA fragments encoding avian cathepsin B were cloned from an osteoclast cDNA library and sequenced. The primary structure of the prepro enzyme deduced from this sequence has 340 amino acids. The mature portion of the enzyme is 80% identical with murine cathepsin B; regions found in other papain superfamily enzymes are conserved. In osteoclasts and cultured macrophages, which produce large quantities of cathepsin B, mRNAs of 1.8 and 2.4 kb are produced in approximately equal quantities, while cells producing smaller quantities of the enzyme produce predominantly the 2.4 kb form. This variation in mRNAs suggests transcriptional differences related to production of large quantities of the enzyme.

引用

页码：69 / 73

页数：5

共 21 条

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