ESTABLISHMENT OF A MONOCLONAL-ANTIBODY RECOGNIZING CYCLOBUTANE-TYPE THYMINE DIMERS IN DNA - A COMPARATIVE-STUDY WITH 64M-1 ANTIBODY SPECIFIC FOR (6-4)PHOTOPRODUCTS

被引：88

作者：

MIZUNO, T

MATSUNAGA, T

IHARA, M

NIKAIDO, O

机构：

[1] KANAZAWA UNIV,FAC PHARMACEUT SCI,DIV RADIAT BIOL,KANAZAWA,ISHIKAWA 920,JAPAN

[2] NARA MED UNIV,DEPT BIOL,KASHIHARA,NARA 634,JAPAN

来源：

MUTATION RESEARCH | 1991年 / 254卷 / 02期

关键词：

MONOCLONAL ANTIBODY; THYMINE DIMER; (6-4)PHOTOPRODUCT; PHOTOREACTIVATION; DNA REPAIR;

D O I：

10.1016/0921-8777(91)90009-E

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We obtained a monoclonal antibody (TDM-1) binding to 313-nm UV-irradiated DNA in the presence of acetophenone. The binding of TDM-1 to 254-nm UV-irradiated DNA was not reduced with the subsequent irradiation of 313-nm UV. Furthermore, the treatment of UV-irradiated DNA with photolyase from E. coli and visible light exposure reduced both the antibody binding and the amount of thymine dimers in the DNA. A competitive inhibition assay revealed that the binding of TDM-1 to UV-irradiated DNA was inhibited with photolyase, but not with 64M-1 antibody specific for (6-4)photoproducts. These results suggest that TDM-1 antibody recognizes cyclobutane-type thymine dimers in DNA. Using TDM-1 and 64M-1 antibodies, we differentially measured each type of damage in DNA extracted from UV-irradiated mammalian cells. Repair experiments confirm that thymine dimers are excised from UV-irradiated cellular DNA more slowly than (6-4)photoproducts, and that the excision rates of thymine dimers and (6-4)photoproducts are lower in mouse NIH3T3 cells than in human cells.

引用

页码：175 / 184

页数：10

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