QUANTIFICATION OF THE HEMATOPORPHYRIN DERIVATIVE BY FLUORESCENCE MEASUREMENT USING DUAL-WAVELENGTH EXCITATION AND DUAL-WAVELENGTH DETECTION

被引：56

作者：

SINAASAPPEL, M ^{[1
]}

STERENBORG, HJCM ^{[1
]}

机构：

[1] UNIV TEXAS, MED BRANCH, BIOMED LASER & SPECT PROGRAM, GALVESTON, TX 77550 USA

来源：

APPLIED OPTICS | 1993年 / 32卷 / 04期

关键词：

HEMATOPORPHYRIN DERIVATIVE; CANCER DETECTION; SPECTROSCOPY; AUTOFLUORESCENCE;

D O I：

10.1364/AO.32.000541

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

A theory is presented describing a method for quantitative measurement of fluorophores in vivo. The method employs a combination of two-wavelength excitation and two-wavelength detection. A dimensionless fluorescence ratio is defined. The theory predicts that this ratio is a function of the concentration of the fluorophore only and is independent of optical properties, independent of geometrical parameters, and independent of autofluorescence. Experiments using a 0.1-10-mug/mL hematoporphyrin derivative in a tissue phantom with the optical properties of the dermis show good agreement with theory.

引用

页码：541 / 548

页数：8

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