COLICIN CLEAVAGE BY OMPT PROTEASE DURING BOTH ENTRY INTO AND RELEASE FROM ESCHERICHIA-COLI-CELLS

被引：31

作者：

CAVARD, D

LAZDUNSKI, C

机构：

[1] Centre de Biochimie, CNRS, 13402 Marseille Cedex 9

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1128/jb.172.2.648-652.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Proteolysis of colicins A, E1, E2, and E3 was observed after they were added to whole cells carrying a functional ompT gene. Recombinant plasmid pML19 containing the ompT gene enabled two mutant strains to cleave the added colicins. On the other hand, two colicin A recombinants were split after release from the wild-type bacteria that produced them but not from ompT mutant cells.

引用

收藏

页码：648 / 652

页数：5

相关论文

共 29 条

[1] EXTRACELLULAR RELEASE OF COLICIN-A IS NONSPECIFIC [J].

BATY, D ;

LLOUBES, R ;

GELI, V ;

LAZDUNSKI, C ;

HOWARD, SP .

EMBO JOURNAL, 1987, 6 (08) :2463-2468

[2] CLEAVAGE OF COLICIN-IA BY THE ESCHERICHIA-COLI K-12 OUTER-MEMBRANE IS NOT MEDIATED BY THE COLICIN-IA RECEPTOR [J].

BOWLES, LK ;

KONISKY, J .

JOURNAL OF BACTERIOLOGY, 1981, 145 (01) :668-671

[3] FRAGMENTATION OF COLICINS A AND E1 BY CELL-SURFACE PROTEASES [J].

BREY, RN .

JOURNAL OF BACTERIOLOGY, 1982, 149 (01) :306-315

[4] POTENTIAL ROLE OF PROTEOLYSIS IN THE CONTROL OF UVRABC INCISION [J].

CARON, PR ;

GROSSMAN, L .

NUCLEIC ACIDS RESEARCH, 1988, 16 (20) :9641-9650

[5] A PROTEASE AS A POSSIBLE SENSOR OF ENVIRONMENTAL-CONDITIONS IN ESCHERICHIA-COLI OUTER-MEMBRANE [J].

CAVARD, D ;

PAGES, JM ;

LAZDUNSKI, CJ .

MOLECULAR & GENERAL GENETICS, 1982, 188 (03) :508-512

[6]

CAVARD D, 1982, FEMS MICROBIOL LETT, V14, P283

[7] HIGH-LEVEL EXPRESSION OF THE COLICIN-A LYSIS PROTEIN [J].

CAVARD, D ;

HOWARD, SP ;

LLOUBES, R ;

LAZDUNSKI, C .

MOLECULAR & GENERAL GENETICS, 1989, 217 (2-3) :511-519

[8]

CAVARD D, 1989, J GEN MICROBIOL, V135, P1715

[9] A MOLECULAR, GENETIC AND IMMUNOLOGICAL APPROACH TO THE FUNCTIONING OF COLICIN-A, A PORE-FORMING PROTEIN [J].

CAVARD, D ;

CROZEL, V ;

GORVEL, JP ;

PATTUS, F ;

BATY, D ;

LAZDUNSKI, C .

JOURNAL OF MOLECULAR BIOLOGY, 1986, 187 (03) :449-459

[10] A RAPID AND CONVENIENT METHOD FOR THE PREPARATION AND STORAGE OF COMPETENT BACTERIAL-CELLS [J].

CHUNG, CT ;

MILLER, RH .

NUCLEIC ACIDS RESEARCH, 1988, 16 (08) :3580-3580