TOWARDS AN ARTIFICIAL CORNEA - SURFACE MODIFICATIONS OF OPTICALLY CLEAR, OXYGEN PERMEABLE SOFT CONTACT-LENS MATERIALS BY AMMONIA PLASMA MODIFICATION TECHNIQUE FOR THE ENHANCED ATTACHMENT AND GROWTH OF CORNEAL EPITHELIAL-CELLS

被引:19
作者
SIPEHIA, R
GARFINKLE, A
JACKSON, WB
CHANG, TMS
机构
[1] Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Quebec, 3655 Druramond Street, Montreal
来源
BIOMATERIALS ARTIFICIAL CELLS AND ARTIFICIAL ORGANS | 1990年 / 18卷 / 05期
关键词
D O I
10.3109/10731199009117333
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The advent of high water content, oxygen permeable contact lens materials has made the intracorneal implants more feasible. A major obstacle encountered is the regrowth of a stable epithelium over the implant. Therefore, ammonia gaseous plasma modification technique was used to modify the surface chemical properties of soft contact lens material such as poly(2-hydroxyethyl methacrylate and methacrylic acid), PHEMA-MAA copolymer, in an attempt to enhance the cell attachment and growth of rabbit corneal epithelial cells. A rabbit corneal epithelial cell line from primary culture was established at Artificial Cells and Organs Research Centre, McGill University. Control (unmodified) and plasma treated surfaces were seeded with corneal epithelium cells. Cell attachment and growth onto these surfaces was studied using 3[H]-labelled amino acids and by light microscopy. Plasma treated surfaces of intraocular lenses (PMMA), polystyrene (PS), and oxygen permeable silicone polymers (silastic membranes) were also evaluated. Enhanced attachment and growth of corneal epithelial cells was observed on ammonia plasma treated surfaces for all materials studied (p values < 0.025).
引用
收藏
页码:643 / 655
页数:13
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