EVIDENCE FOR A MEMBRANE SKELETON IN HIGHER-PLANTS - A SPECTRIN-LIKE POLYPEPTIDE CO-ISOLATES WITH RICE ROOT PLASMA-MEMBRANES

被引：41

作者：

FARADAY, CD

SPANSWICK, RM

机构：

[1] Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca

来源：

FEBS LETTERS | 1993年 / 318卷 / 03期

关键词：

AQUEOUS POLYMER 2-PHASE PARTITIONING; FODRIN; PERIPHERAL PROTEIN; CYTOSKELETON PLASMA MEMBRANE INTERACTION;

D O I：

10.1016/0014-5793(93)80536-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fraction enriched in plasma membranes was isolated from rice roots by differential centrifugation and aqueous polymer two-phase partitioning. Analysis of the fraction by SDS-PAGE showed the presence of several low mobility polypeptides (M(r)> 100 kDa). One of these polypeptides (M(r) approximately 230 kDa) was specifically recognized by polyclonal antibodies to human erythrocyte spectrin. This finding suggests that a higher plant spectrin-based membrane skeleton may be preserved and studied using high-purity plasma membrane fractions obtained by aqueous polymer two-phase partitioning.

引用

页码：313 / 316

页数：4

共 20 条

[1] THE SPECTRIN SKELETON - FROM RED-CELLS TO BRAIN [J].