CHEMICAL SYNTHESIS OF C-13-LABELED MONOMERS FOR THE SOLID-PHASE AND TEMPLATE CONTROLLED ENZYMATIC-SYNTHESIS OF DNA AND RNA OLIGOMERS

The preparation of C-13-labelled ribonucleosides starting from [C-13(6)]-glucose 1 and the corresponding nucleobases 5a-e or 6a-e (N-6-benzoyl-adenine, N-2-acetyl-guanine, N-4-benzoyl-cytosine, uracil and thymine) in 47 - 66% overall yield is described. Their subsequent transformation into 5'-O-dimethoxytrityl protected DNA-phosphoramidites and 5'-O-dimethoxytrityl-2'-O-trialkylsilyl protected RNA-phosphor-amidites for the solid phase synthesis of DNA- and RNA-oligomers and to 5'-O-ribo- and deoxyribo-nucleosidetriphosphates for template controlled enzymatic synthesis (polymerase- or reverse transcriptase reaction) has been carried out.