MECHANISM OF ACTIVE DNA DEMETHYLATION DURING EMBRYONIC-DEVELOPMENT AND CELLULAR-DIFFERENTIATION IN VERTEBRATES

被引：19

作者：

JOST, JP

JOST, YC

机构：

[1] Friedrich Miescher Institut

来源：

GENE | 1995年 / 157卷 / 1-2期

关键词：

ADP-RIBOSYLATION; DNA POLYMERASE BETA; HEMIMETHYLATED DNA; M(5)CPG ENDONUCLEASE; METHYLTRANSFERASE;

D O I：

10.1016/0378-1119(95)00036-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Incubation of hemimethylated and labelled oligodeoxynucleotides with nuclear extracts from differentiating chicken embryos and mouse myoblasts resulted in the replacement of m(5)C by C. One of the enzymes involved is m(5)CpG endonuclease. It cleaves only m(5)CpG and not, m(5)CpT, m(5)CpA, m(5)CpC or m(6)ApT. The enzyme is not sequence specific and catalyses the reaction in the presence of high concentrations of EDTA or EGTA.

引用

页码：265 / 266

页数：2

共 15 条

[1] DNA DEMETHYLATION IN ERYTHROLEUKEMIA-CELLS [J].