NITRATE REDUCTASE FROM SPINACEA-OLERACEA FAD AND INACTIVATION BY NAD(P)H

被引：2

作者：

CASTILLO, F ^{[1
]}

DELAROSA, FF ^{[1
]}

CALERO, F ^{[1
]}

PALACIAN, E ^{[1
]}

机构：

[1] UNIV SEVILLA, CONSEJO SUPERIOR INVEST CIENT, FAC CIENCIAS, DEPT BIOQUIMICA, Seville, SPAIN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1976年 / 69卷 / 02期

关键词：

D O I：

10.1016/0006-291X(76)90518-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Treatment by P-hydroxymercuribenzoate of nitrate reductase from spinach leaves caused the disappearance of NADH-diaphorase activity and the appearance of an FAD-requirement for the inactivation by NAD(P)H of FNH2-nitrate reductase. The diaphorase activity of the treated preparation was not affected by incubation with FAD or the addition of this nucleotide to the assay mixture. Conversely, filtration of the native preparation through a column of Sepharose 6B produced the appearance of an FAD-stimulation of the diaphorase activity, but no effect of FAD on the NADH-inactivation was observed. These differences between the FAD-requirement of NADH-diaphorase activity and NADH-inactivation agreed with the postulated independence of the 2 processes.

引用

页码：277 / 284

页数：8

共 8 条

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