SEPARATION OF METHANOTROPHIC BACTERIA BY USING PERCOLL AND ITS APPLICATION TO ISOLATION OF MIXED AND PURE CULTURES

被引：16

作者：

PUTZER, KP

BUCHHOLZ, LA

LIDSTROM, ME

REMSEN, CC

机构：

[1] UNIV WISCONSIN,CTR GREAT LAKES STUDIES,MILWAUKEE,WI 53201

[2] UNIV WISCONSIN,DEPT BIOL SCI,MILWAUKEE,WI 53201

[3] CALTECH,KECK LABS,PASADENA,CA 91125

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 12期

关键词：

D O I：

10.1128/AEM.57.12.3656-3659.1991

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Type I and II methanotrophic bacteria can be cleanly separated by using Percoll buoyant density gradients. This simple and inexpensive method can be used to screen new isolates rapidly for preliminary classification. In addition, Percoll gradients can also be used to enhance standard enrichment techniques for methanotrophs from natural water samples.

引用

页码：3656 / 3659

页数：4

共 16 条

[1] ANTHONY C, 1982, BIOCH METHYLOTROPHS, P3
[2] SEPARATION OF SYNTROPHOMONAS-WOLFEI FROM METHANOSPIRILLUM-HUNGATII IN SYNTROPHIC COCULTURES BY USING PERCOLL GRADIENTS
BEATY, PS
WOFFORD, NQ
MCINERNEY, MJ
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1987, 53 (05) : 1183 - 1185
[3] Guerrero R, 1985, Microbiologia, V1, P53
[4] ISOLATION AND CHARACTERIZATION OF MARINE METHANOTROPHS
LIDSTROM, ME
[J]. ANTONIE VAN LEEUWENHOEK JOURNAL OF MICROBIOLOGY, 1988, 54 (03): : 189 - 199
[5] TRICHLOROETHYLENE BIODEGRADATION BY A METHANE-OXIDIZING BACTERIUM
LITTLE, CD
PALUMBO, AV
HERBES, SE
LIDSTROM, ME
TYNDALL, RL
GILMER, PJ
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (04) : 951 - 956
[6] SEPARATION OF NON-FILAMENTOUS MICROORGANISMS FROM SOIL BY DENSITY GRADIENT CENTRIFUGATION IN PERCOLL
MARTIN, NJ
MACDONALD, RM
[J]. JOURNAL OF APPLIED BACTERIOLOGY, 1981, 51 (02): : 243 - 251
[7] SPECIFIC BINDING OF RAT-LIVER PLASMA-MEMBRANES BY RAT-LIVER CELLS
OBRINK, B
WARMEGARD, B
PERTOFT, H
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1977, 77 (02) : 665 - 670
[8] PERTOF H, 1979, BIOCHEMISTRY-US, V9, P67
[9] Pertoft H., 1977, METHODS CELL, P25
[10] HARVEST OF PLANKTONIC MARINE ALGAE BY CENTRIFUGATION INTO GRADIENTS OF SILICA IN CF-6 CONTINUOUS-FLOW ZONAL ROTOR
PRICE, CA
MENDIOLA.LR
GOLDSTEIN, M
BREDEN, EN
GUILLARD, RR
[J]. BIOLOGICAL BULLETIN, 1974, 147 (01) : 136 - 145

← 1 2 →