ISOLATION AND CHARACTERIZATION OF AN NDVB LOCUS FROM RHIZOBIUM-FREDII

被引：31

作者：

BHAGWAT, AA ^{[1
]}

TULLY, RE ^{[1
]}

KEISTER, DL ^{[1
]}

机构：

[1] USDA ARS,BELTSVILLE AGR RES CTR,SOYBEAN & ALFALFA RES LAB,BLDG 011,HH-19,BARC W,BELTSVILLE,MD 20705

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 15期

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01389.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene (ndvB) in Rhizobium meliloti that is essential for nodule development in Medicago sativa (alfalfa), specifies synthesis of a large membrane protein. This protein appears to be an intermediate in beta-1,2-glucan synthesis by the microsymbiont. Southern hybridization analysis showed strong homology between an ndvB (chvB) probe and genomic DNA of R. fredii but not from Bradyrhizobium japonicum. A cosmid clone containing the putative ndvB locus was isolated from a Rhizobium fredii gene library. The cosmid clone which complemented R. meliloti ndvB mutants for synthesis of beta-1,2-glucans and effective nodulation of alfalfa was mapped and subcloned. Fragment-specific Tn5 mutagenesis followed by homologous recombination into the R. fredii genome indicated that the region was essential for beta-1,2-glucan synthesis and for formation of an effective symbiosis with Glycine max (soybean).

引用

页码：2159 / 2165

页数：7

共 29 条

[1] STUDIES ON CYCLIC BETA-1,2-GLUCAN OBTAINED FROM PERIPLASMIC SPACE OF RHIZOBIUM-TRIFOLII CELLS [J].