SITE OF DOPAMINE D-1 RECEPTOR-BINDING TO G(S) PROTEIN MAPPED WITH SYNTHETIC PEPTIDES

被引：21

作者：

KONIG, B

GRATZEL, M

机构：

[1] Ecole Polytechnique Fédérale de Lausanne Instiut de Chimie Physique II

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH | 1994年 / 1223卷 / 02期

关键词：

DOPAMINE D-1 RECEPTOR; G(S) PROTEIN; BINDING SITE; SYNTHETIC PEPTIDE; RECEPTOR BINDING;

D O I：

10.1016/0167-4889(94)90235-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have synthesized peptides corresponding to the known sequence of the rat dopamine D-1 receptor and measured the effects on binding of the G(s) protein to the activated receptor. Peptides corresponding to the second and third cytoplasmic loop (amino acids 160-180 and 260-276), as well as the N-terminal part of the carboxy terminus (amino acids 375-391) were effective in preventing G(s) binding to the activated receptor. Peptides representing other surface regions, e.g., the first cytoplasmic loop (87-100), the C-terminus (400-420 and 440-460) and all extracellular surface peptides (6-22, 120-137, 210-224 and 338-352) are not involved in rat dopamine D-1 receptor-G(s) protein interaction.

引用

页码：261 / 266

页数：6

共 24 条

[1] DOPAMINE RECEPTOR SUBTYPES - BEYOND THE D1/D2 CLASSIFICATION [J].