H-1 AND C-13 NMR CHEMICAL-SHIFTS OF THE DIASTEREOTOPIC METHYL-GROUPS OF VALYL AND LEUCYL RESIDUES IN PEPTIDES AND PROTEINS

被引：31

作者：

NERI, D ^{[1
]}

OTTING, G ^{[1
]}

WUTHRICH, K ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL, INST MOLEK BIOL & BIOPHYS, CH-8093 ZURICH, SWITZERLAND

来源：

TETRAHEDRON | 1990年 / 46卷 / 09期

关键词：

D O I：

10.1016/S0040-4020(01)85464-8

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Stereospecific 1H and 13C NMR assignments for the diastereo-topic methyl groups of the valyl and leucyl residues in a cyclic peptide and in two globular proteins were obtained using biosynthetically direc-ted fractional isotope labeling with 13C. The methyl chemical shifts are analyzed with respect to possible correlations with the stereochemistry of these amino acid side chains. © 1990.

引用

页码：3287 / 3296

页数：10

共 30 条

[1]

BAX A, 1989, ANNU REV BIOCHEM, V58, P223

[2] NATURAL ABUNDANCE N-15 NMR BY ENHANCED HETERONUCLEAR SPECTROSCOPY [J].

BODENHAUSEN, G ;

RUBEN, DJ .

CHEMICAL PHYSICS LETTERS, 1980, 69 (01) :185-189

[3] DETERMINATION OF 3-DIMENSIONAL STRUCTURES OF PROTEINS AND NUCLEIC-ACIDS IN SOLUTION BY NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY [J].

CLORE, GM ;

GRONENBORN, AM .

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1989, 24 (05) :479-564

[4] BIOSYNTHESIS OF L-VALINE IN SALMONELLA-TYPHIMURIUM - ORIGIN OF THE DIASTEREOTOPIC METHYL-GROUPS [J].

CROUT, DHG ;

HEDGECOCK, CJR ;

LIPSCOMB, EL ;

ARMSTRONG, FB .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1980, (07) :304-305

[5] THE INFLUENCE OF STEREOSPECIFIC ASSIGNMENTS ON THE DETERMINATION OF 3-DIMENSIONAL STRUCTURES OF PROTEINS BY NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY - APPLICATION TO THE SEA-ANEMONE PROTEIN BDS-I [J].

DRISCOLL, PC ;

GRONENBORN, AM ;

CLORE, GM .

FEBS LETTERS, 1989, 243 (02) :223-233

[6]

Ernst R. R., 1987, PRINCIPLES NUCL MAGN, V1st

[7]

GUNTERT P, 1989, J AM CHEM SOC, V111, P3997

[8] ENZYMATIC DISCRIMINATION BETWEEN DIASTEREOTOPIC ENOL FACES IN DEHYDRASE STEP OF VALINE BIOSYNTHESIS [J].

HILL, RK ;

YAN, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1973, 95 (23) :7857-7859

[9] STEREOCHEMISTRY OF VALINE AND ISOLEUCINE BIOSYNTHESIS .4. SYNTHESIS, CONFIGURATION, AND ENZYMATIC SPECIFICITY OF ALPHA-ACETOLACTATE AND ALPHA-ACETO-ALPHA-HYDROXYBUTYRATE [J].

HILL, RK ;

SAWADA, S ;

ARFIN, SM .

BIOORGANIC CHEMISTRY, 1979, 8 (02) :175-189

[10] PROTEIN STRUCTURES FROM NMR [J].

KAPTEIN, R ;

BOELENS, R ;

SCHEEK, RM ;

VANGUNSTEREN, WF .

BIOCHEMISTRY, 1988, 27 (15) :5389-5395

← 1 2 3 →