SIMULTANEOUS MEASUREMENT OF NEUTROPHIL, LYMPHOCYTE, AND MONOCYTE GLUTATHIONE BY FLOW-CYTOMETRY

被引：19

作者：

SCOTT, RB

COLLINS, JM

MATIN, S

WHITE, F

SWERDLOW, PS

机构：

[1] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT PATHOL,RICHMOND,VA 23298

[2] VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT BIOCHEM & MOLEC BIOPHYS,RICHMOND,VA 23298

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 1990年 / 4卷 / 05期

关键词：

fluorescence; Leukocytes; light scatter; monochlorobimane; peripheral blood;

D O I：

10.1002/jcla.1860040503

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominately GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols closely reflected the GSH content, as measured by a specific glutathione reductase assay. Fluorescence of individual cell types could be measured after delineating those cells by their light‐scatter characteristics, utilizing dual‐angle light scatter for discrimination. By this means, GSH contents of 12.5 ± 2.0nmol/107 neutrophils, 14.5 ± 2.7 nmol/107 monocytes, and 5.0 ± 1.0 nmol/107 lymphocytes were found. The results obtained for neutrophils with the flow cytometer were virtually identical with those obtained with chemical assay in purified samples of neutrophils, indicating the validity of the flow cytometric method. Copyright © 1990 Wiley Periodicals, Inc., A Wiley Company

引用

页码：324 / 327

页数：4

共 17 条

[1] EFFECT OF HALOTHANE AND ENFLURANE ANESTHESIA ON THE LEVEL OF REDUCED GLUTATHIONE IN HUMAN RED-BLOOD-CELLS [J].