ENDOTHELIN RELEASING ACTIVITY IN CALF SERUM AND PORCINE FOLLICULAR-FLUID

被引:13
作者
BROWN, MR
VAUGHAN, J
WALSH, J
JIMENEZ, L
HEXUM, TD
BAIRD, A
VALE, W
机构
[1] UNIV CALIF SAN DIEGO, DEPT SURG, SAN DIEGO, CA 92103 USA
[2] SALK INST BIOL STUDIES, CLAYTON FDN LABS PEPTIDE BIOL, LA JOLLA, CA 92037 USA
[3] UNIV NEBRASKA, MED CTR, DEPT PHARMACOL, OMAHA, NE 68198 USA
[4] SCRIPPS CLIN & HOSP, WHITTIER INST, LA JOLLA, CA 92037 USA
关键词
D O I
10.1016/S0006-291X(05)80859-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bovine calf serum (CS) and porcine follicular fluid (PFF) added to bovine aortic endothelial cells (BAEC) in culture stimulate endothelin (ET) release. Sorbent extraction of acidified calf serum and PFF using Bond Elut C18 yields a product with an activity 30% that of the starting material. Fractionation of Bond Elut C18 extracted CS or PFF using HPLC yields peaks of activity with retention times similar to those of TGFβ and activin A, respectively. TGFβ is 10-fold more potent than activin A to stimulate ET release from BAECs. The observation that TGFβ and activin A are dose-additive but not effect-additive to stimulate ET release is compatible with the conclusion that these two substances act through a similar mechanism. Thus, TGFβ and/or activin A-related peptides may contribute to the ET releasing activity observed in acidified Bond Elut C18 extracted CS and PFF. The identity of ET-releasing activity in native CS and PFF remains to be established. © 1990 Academic Press, Inc.
引用
收藏
页码:807 / 815
页数:9
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