INCREASED HYALURONAN FLUX FROM SKIN FOLLOWING BURN INJURY

Hyaluronan (formerly hyaluronic acid) is an important constituent of the interstitial matrix in skin. Following major burn injury in animal models, plasma hyaluronan can increase to levels 10-fold greater than normal. The present experiments were designed to determine whether this is a result of the increased lymph flow (Q̇L) accompanying the injury or of an increased release of hyaluronan from the burned skin and subcutaneous tissue. The lateral saphenous vein and a prenodal lymphatic were cannulated in the hindpaw of five anesthetized canines. Hindpaw venous pressure was elevated until the total protein concentration in lymph declined to steady-state levels, and Q̇L and hyaluronan flux (Q̇L × [hyaluronan]) was measured. A minor burn was inflicted by immersion of the paw into 100°C water for five sec, and measurements were repeated at regular intervals for a minimum of 4 hr. Burn injury resulted in significant and persistent increases in Q̇L (154 ± 61 μl/min versus 562 ± 105 μl/min 4 hr postburn) and lymph total protein concentration (1.34 ± 0.04 g/dl versus 4.08 ± 0.18 g/dl 4 hr postburn), while lymph hyaluronan concentration fell (3.01 ± 0.20 μg/ml versus 2.1 ± 0.16 μg/ml 4 hr postburn). The resultant increase in hyaluronan flux (0.42 ± 0.13 μg/min versus 1.17 ± 0.22 μg/min 4 hr postburn) appears to be a function of lymph flow rather than burn-induced release of skin hyaluronan. Hence, the increased plasma concentration of hyaluronan following major burns is likely a consequence of increased lymph flow from the site of injury. © 1991.