ANALYSIS OF THE NEUROTOXIN BETA-ODAP AND ITS ALPHA-ISOMER BY PRECOLUMN DERIVATIZATION WITH PHENYLISOTHIOCYANATE

被引：33

作者：

KHAN, JK

KEBEDE, N

KUO, YH

LAMBEIN, F

DEBRUYN, A

机构：

[1] STATE UNIV GHENT,FAC MED,PHYSIOL CHEM LAB,LEDEGANCKSTR 35,B-9000 GHENT,BELGIUM

[2] STATE UNIV GHENT,ORGAN CHEM LAB,B-9000 GHENT,BELGIUM

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 208卷 / 02期

关键词：

D O I：

10.1006/abio.1993.1038

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is presented for determining 3-N-oxalyl-2,3-diaminopropanoic acid (β-ODAP), the most potent neurotoxic substance of the seeds and seedlings of Lathyrus sativus, and its much less toxic 2-isomer (α-ODAP). The separation of the two forms is achieved after derivatization with phenylisothiocyanate employing a HPLC system. This method was used to monitor the isomerization of β-ODAP to α-ODAP at different time intervals and to quantify the toxin level in seed extracts. © 1994 Academic Press, Inc. All rights reserved.

引用

页码：237 / 240

页数：4

共 15 条

[1] ISOLATION OF ALPHA- AND GAMMA-OXALYL DERIVATIVES OF ALPHA,GAMMA-DIAMINOBUTYRIC ACID FROM SEEDS OF LATHYRUS LATIFOLIUS AND DETECTION OF ALPHA-OXALYL ISOMER OF NEUROTOXIN ALPHA-AMINO-BETA-OXALYL-AMINOPROPIONIC ACID WHICH OCCURS TOGETHER WITH NEUROTOXIN IN THIS AND OTHER SPECIES [J].