COMBINED STAINING METHOD FOR THE DEMONSTRATION OF TISSUE EOSINOPHILS AND MAST-CELLS

被引：24

作者：

DUFFY, JP ^{[1
]}

SMITH, PJ ^{[1
]}

CROCKER, J ^{[1
]}

MATTHEWS, HR ^{[1
]}

机构：

[1] E BIRMINGHAM DIST GEN HOSP,DEPT HISTOPATHOL,BIRMINGHAM B9 5ST,W MIDLANDS,ENGLAND

来源：

JOURNAL OF HISTOTECHNOLOGY | 1993年 / 16卷 / 02期

关键词：

ASTRA BLUE STAIN; EOSINOPHILS; MAST CELLS; VITAL NEW RED STAIN;

D O I：

10.1179/his.1993.16.2.143

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Quantitation of mast cells and eosinophils is of value in studies of allergic or parasitic tissue inflammation. We describe a new method that combines two reliable stains, astra blue and vital new red, to stain both mast cells and eosinophils in the same tissue section. The mast cells stain violet-blue and the eosinophils bright red. Because this method stains both cells in tissue that has been fixed with formol saline or buffered formaldehyde, it can be used on archival tissue that has undergone standard fixation. The minimal staining of the background makes quantitative studies of both cells simple, even at low magnification.

引用

页码：143 / 144

页数：2

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