MORPHOLOGY IN INTRACYTOPLASMIC SPERM INJECTION - PRELIMINARY-RESULTS

被引：37

作者：

KUPKER, W

ALHASANI, S

SCHULZE, W

KUHNEL, W

SCHILL, T

FELBERBAUM, R

机构：

[1] Department of Obstetrics/Gynecology, Medical University Lübeck, Lübeck, 23562

[2] Department of Andrology, University Hospital Eppendorf, Hamburg

[3] Institute of Anatomy, Medical University Lübeck

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1995年 / 12卷 / 09期

关键词：

ICSI; OAT SYNDROME; ULTRASTRUCTURE OF SPERMATOZOA AND OOCYTES;

D O I：

10.1007/BF02212586

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: Direct intracytoplasmic sperm injection (ICSI) is a successful treatment of severe male subfertility. Conventional in vitro fertilization shows poor pregnancy rates especially in cases of severe teratozoospermia, The objective was to determine if severe morphological defects of spermatozoa in oligoasthenoteratozoospermia (OAT) have any impact on the fertilization process in ICSI and if there are any specific morphological characteristics in nonfertilized oocytes after the ICSI procedure, Methods: Ejaculates and nonfertilized oocytes were investigated by electron microscopy. Conclusions: The lack of intracytoplasmic sperm-oocyte interaction, not severe sperm defects, displays the most critical role in the fertilization process. Clinical data with fertilization rates of 66% and pregnancy rates of 23.3% confirm the fertilization capacity of severely defective spermatozoa in ICSI.

引用

页码：620 / 626

页数：7

共 4 条

[1]

Alpustun S., Al-Hasani S., Diedrich K., Bauer O., Werner A., Krebs D., In-vitro-Fertilisation Prognostische Faktoren, Geburtsh Frauenheilk, 53, pp. 351-355, (1993)

[2]

Kruger T.F., Menkveld R., Stander F.S.H., Lombard C.J., Van der Merwe J.P., Van Zyl J.A., Smith K., Sperm morphologic features as a prognostic factor in in vitro ferilization, Fertil Steril, 46, pp. 1118-1123, (1986)

[3]

Ombelet W., Fourie, Vandeput H., Bosmans E., Cox A., Janssen M., Kruger T., Teratozoospermia and in-vitro fertilization: a randomized prospective study, Hum Reprod, 9, pp. 1479-1484, (1994)

[4]

Van Blerkom J., Extrinsic and intrinsic influences on human oocyte and early embryo developmental potential, Elements of Mammalian Fertilization. Vol. II, pp. 81-109, (1991)

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