THE INFECTIVITY OF DIMERIC POTATO YELLOW MOSAIC GEMINIVIRUS CLONES IN DIFFERENT HOSTS

被引：18

作者：

BURAGOHAIN, AK ^{[1
]}

SUNG, YK ^{[1
]}

COFFIN, RS ^{[1
]}

COUTTS, RHA ^{[1
]}

机构：

[1] UNIV LONDON IMPERIAL COLL SCI TECHNOL & MED,DEPT BIOL,LONDON SW7 2BB,ENGLAND

来源：

JOURNAL OF GENERAL VIROLOGY | 1994年 / 75卷

关键词：

D O I：

10.1099/0022-1317-75-10-2857

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Head-to-tail dimeric clones of both DNA A and DNA B of potato yellow mosaic geminivirus (PYMV) were constructed. These constructs were infectious when inoculated onto Nicotiana benthamiana plants either as DNA or by agroinoculation and were also infectious for tomato plants by agroinoculation. The dimers were not infectious for potato plants following inoculation by either method. Symptom induction required both DNA A and DNA B but agroinoculation with DNA A alone resulted in virus spread in 30% of the inoculated N. benthamiana plants. Leaf disc explants of N. benthamiana, tomato and potato could all be infected by agroinoculation indicating that the method of delivery of the DNA to intact potato plants was unsuitable for successful inoculation rather than an inherent inability of the virus to replicate/spread in potato per se. Neither whole plants nor leaf discs of sugar beet supported the replication of PYMV DNA.

引用

页码：2857 / 2861

页数：5

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