IDENTIFICATION OF DNA TOPOISOMERASES INVOLVED IN IMMEDIATE AND TRANSIENT DNA RELAXATION INDUCED BY HEAT-SHOCK IN ESCHERICHIA-COLI

被引：34

作者：

OGATA, Y ^{[1
]}

MIZUSHIMA, T ^{[1
]}

KATAOKA, K ^{[1
]}

MIKI, T ^{[1
]}

SEKIMIZU, K ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC PHARMACEUT SCI,DEPT MICROBIOL,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

MOLECULAR & GENERAL GENETICS | 1994年 / 244卷 / 05期

关键词：

HEAT SHOCK; DNA RELAXATION; TOPA DNA TOPOISOMERASE I; DNA GYRASE;

D O I：

10.1007/BF00583895

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The linking number of plasmid DNA in exponentially growing Escherichia coli increases immediately and transiently after heat shock. The purpose of this study was to search for DNA topoisomerases that catalyze this relaxation of DNA. Neither introduction of a topA deletion mutation nor treatment of cells with DNA gyrase inhibitors affected the DNA relaxation induced by heat shock. Thus, DNA topoisomerase I and DNA gyrase are apparently not involved in the process. However, the reaction was inhibited by nalidixic acid or by oxolinic acid in the topA mutant and the reaction was resistant to nalidixic acid in a topA mutant carrying, in addition, the nalA26 mutation. These results are interpreted as indicating that both DNA topoisomerase I and DNA gyrase are involved in the DNA relaxation induced by heat shock.

引用

页码：451 / 455

页数：5

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