AN IN-VITRO BIOASSAY FOR NERVE GROWTH-FACTOR BASED ON 24-HOUR SURVIVAL OF PC-12 CELLS

被引：18

作者：

ROBINSON, CJ

STAMMERS, R

机构：

[1] National Institute for Biological Standards and Control, Potters Bar, Hertfordshire, EN6 3QG, Blanche Lane, South Mimms

来源：

GROWTH FACTORS | 1994年 / 10卷 / 03期

关键词：

NERVE GROWTH FACTOR; PC-12; IN VITRO BIOASSAY;

D O I：

10.3109/08977199409000237

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have developed an in vitro bioassay for nerve growth factor (NGF) which offers several advantages over currently used alternatives. The assay uses the commercially available PC-12 cell line, and requires only 24 hr incubation with NGF. The cells do not require priming, and are dosed as they are originally seeded in serum-free medium in 96-well cell culture plates coated with collagen. Concentrations between one and 20 ng/ml NGF can be measured, and only standard cell culture equipment and a microplate reader are required.

引用

页码：193 / 196

页数：4

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