CLONING AND SEQUENCING OF MAL-D-1, THE MAJOR ALLERGEN FROM APPLE (MALUS-DOMESTICA), AND ITS IMMUNOLOGICAL RELATIONSHIP TO BET-V-1, THE MAJOR BIRCH POLLEN ALLERGEN

被引：247

作者：

VANEKKREBITZ, M

HOFFMANNSOMMERGRUBER, K

MACHADO, MLD

SUSANI, M

EBNER, C

KRAFT, D

SCHEINER, O

BREITENEDER, H

机构：

[1] UNIV VIENNA,INST GEN & EXPTL PATHOL,A-1090 VIENNA,AUSTRIA

[2] AGR UNIV VIENNA,INST APPL MICROBIOL,A-1180 VIENNA,AUSTRIA

[3] ACAD SCI SALZBURG,INST MOLEC BIOL,SALZBURG,AUSTRIA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 214卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.2320

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The number of tree - pollen-allergic patients showing allergic reactions to apples, various vegetables and nuts is increasing. In this paper the molecular characterization of the major apple allergen, Mal d 1, is reported. The cDNA coding for Mal d 1 was cloned and sequenced. Its open reading frame codes for a protein of 159 amino acids with a predicted molecular mass of 17.7 kDa and a predicted pI of 5.9. Sequence comparison to Bet v 1, the major birch pollen allergen, revealed 64.5% identity on the amino acid level and 55.6% identity on the nucleic acid level. Recombinant Mal d 1 was expressed in the plasmid pMW 175 in E. coli BL 21 (DE3) and its immunological properties were tested, Crossreactivity with Bet v 1 was shown by inhibition assays. (C) 1995 Academic Press, Inc.

引用

收藏

页码：538 / 551

页数：14

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