EFFECT OF DIFFERENT FIXATIVES ON CON-A SURFACE-RECEPTORS OF MOUSE PERITONEAL-MACROPHAGES

被引：11

作者：

PESCHKE, T ^{[1
]}

WOLLWEBER, L ^{[1
]}

GABERT, A ^{[1
]}

AUGSTEN, K ^{[1
]}

STRACKE, R ^{[1
]}

机构：

[1] INST BIOTECHNOL,O-7028 LEIPZIG,GERMANY

来源：

HISTOCHEMISTRY | 1990年 / 93卷 / 04期

关键词：

D O I：

10.1007/BF00315865

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effects of glutaraldehyde, formaldehyde, or osmium tetroxide fixation on the number of labeled Con A surface receptors on mouse peritoneal macrophages were compared. Gold-labeled Con A receptors were found to be isolatedly arranged and evenly distributed on cell surfaces independent of the fixative used. Only cells preincubated with Con A and subsequently fixed by osmium tetroxide showed arrangement of labeled receptors in clusters. Significant differences were found in the number of Con A receptors per cell depending on the fixative used. The fluorescence intensity of FITC-Con A staining was detected spectrophotometrically, the characteristic X-rays of gold-labeled Con A receptors were determined by means of electron beam-induced X-ray microanalysis. The experimental results obtained both at light and electron microscopic level pointed to formaldehyde being the best fixative also for this purpose. © 1990 Springer-Verlag.

引用

页码：443 / 446

页数：4

共 29 条

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