ACTIVATION BY CROMAKALIM OF PRESYNAPTIC AND POSTSYNAPTIC ATP-SENSITIVE K+ CHANNELS IN SUBSTANTIA-NIGRA

被引：78

作者：

HAUSSER, MA ^{[1
]}

DEWEILLE, JR ^{[1
]}

LAZDUNSKI, M ^{[1
]}

机构：

[1] UNIV OXFORD,PHYSIOL LAB,OXFORD OX1 3PT,ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91504-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Membrane potentials and synaptic potentials were recorded using the patch clamp technique from neurons isolated from the substantia nigra. Intracellular perfusion of dopaminergic neurons with an ATP-free solution caused hyperpolarization and inhibition of firing. Intracellular perfusion with a solution containing 2 mM ATP prevented this hyperpolarization, but application of the K+ channel openers cromakalim and pinacidil caused a similar hyperpolarization as well as the disappearance of bicuculline-sensitive synaptic potentials. All these effects were reversed by sulfonylureas, indicating that they are mediated by ATP-sensitive K+ channels. It is concluded that K+ channel openers activate ATP-sensitive K+ channels both presynaptically on GABAergic terminals and postsynaptically on substantia nigra dopaminergic neurons. © 1991.

引用

页码：909 / 914

页数：6

共 29 条

[1] POTASSIUM CHANNEL ACTIVATORS ABOLISH EXCITOTOXICITY IN CULTURED HIPPOCAMPAL PYRAMIDAL NEURONS [J].