DEOXYNUCLEOTIDE POOLS AND DNA-SYNTHESIS IN RESTING AND PHA-STIMULATED HUMAN-LYMPHOCYTES TREATED WITH MUTAGENS

被引：15

作者：

FERRARO, P ^{[1
]}

BIANCHI, V ^{[1
]}

BIASIN, MR ^{[1
]}

CELOTTI, L ^{[1
]}

机构：

[1] UNIV PADUA,DEPT BIOL,VIA TRIESTE 75,I-35121 PADUA,ITALY

来源：

EXPERIMENTAL CELL RESEARCH | 1992年 / 199卷 / 02期

关键词：

D O I：

10.1016/0014-4827(92)90444-D

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In resting and PHA-stimulated PBL treated with uv light or MMS we measured the sizes of the dTTP and dATP pools and the variation of ATP content taken as an indicator of cytotoxicity. The effects on DNA synthesis were examined by measuring DNA repair (unscheduled DNA synthesis) in resting PBL and the inhibition of DNA replication in stimulated PBL. While both treatments affected DNA synthesis, only MMS perturbed dNTP pools and decreased the intracellular concentration of ATP. All the effects were more evident in cycling than in resting lymphocytes. © 1992.

引用

页码：349 / 354

页数：6

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