MAP-BASED CLONING OF A PROTEIN-KINASE GENE CONFERRING DISEASE RESISTANCE IN TOMATO

被引：1104

作者：

MARTIN, GB ^{[1
]}

BROMMONSCHENKEL, SH ^{[1
]}

CHUNWONGSE, J ^{[1
]}

FRARY, A ^{[1
]}

GANAL, MW ^{[1
]}

SPIVEY, R ^{[1
]}

WU, TY ^{[1
]}

EARLE, ED ^{[1
]}

TANKSLEY, SD ^{[1
]}

机构：

[1] CORNELL UNIV, DEPT PLANT BREEDING & BIOMETRY, ITHACA, NY 14853 USA

来源：

SCIENCE | 1993年 / 262卷 / 5138期

关键词：

D O I：

10.1126/science.7902614

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The Pto gene in tomato confers resistance to races of Pseudomonas syringae pv. tomato that carry the avirulence gene avrPto. A yeast artificial chromosome clone that spans the Pto region was identified and used to probe a leaf complementary DNA (cDNA) library. A cDNA clone was isolated that represents a gene family, at least six members of which genetically cosegregate with Pto. When susceptible tomato plants were transformed with a cDNA from this family, they were resistant to the pathogen. Analysis of the amino acid sequence revealed similarity to serine-threonine protein kinases, suggesting a role for Pto in a signal transduction pathway.

引用

页码：1432 / 1436

页数：5

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