ANNEXIN-I INHIBITS PHOSPHOLIPASE A(2) BY SPECIFIC INTERACTION, NOT BY SUBSTRATE DEPLETION

被引：119

作者：

KIM, KM

KIM, DK

PARK, YM

KIM, CK

NA, DS

机构：

[1] UNIV ULSAN,COLL MED,DEPT BIOCHEM,SEOUL,SOUTH KOREA

[2] SEOUL NATL UNIV,COLL PHARM,SEOUL,SOUTH KOREA

来源：

FEBS LETTERS | 1994年 / 343卷 / 03期

关键词：

PHOSPHOLIPASE A(2); ANNEXIN-I; INHIBITION MECHANISM; SUBSTRATE DEPLETION; SPECIFIC INTERACTION;

D O I：

10.1016/0014-5793(94)80566-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Annexin-I is a calcium dependent phospholipid binding and phospholipase A(2) (PLA(2)) inhibitory protein. A 'substrate depletion' model has been proposed for the mechanism of PLA, inhibition by annexin-I in studies with 14 to 18 kDa PLA(2)s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA(2) from porcine spleen. The inhibition has been measured at various substrate and calcium ion concentrations. The pattern of PLA(2) inhibition by annexin-I was consistent with a 'specific interaction' mechanism rather than the 'substrate depletion' model. Apparent contradiction with previous studies can be explained by the calcium-dependent binding of annexin-I to the substrate.

引用

页码：251 / 255

页数：5

共 24 条

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