T-CELL BLASTOGENIC RESPONSES TO TOXOPLASMA-GONDII TROPHOZOITES AMONG HIV-INFECTED PATIENTS

被引:4
作者
CARREGA, G [1 ]
CANESSA, A [1 ]
ARGENTA, P [1 ]
CRUCIANI, M [1 ]
BASSETTI, D [1 ]
机构
[1] UNIV GENOA, DEPT INFECT DIS, I-16132 GENOA, ITALY
关键词
D O I
10.1089/aid.1995.11.741
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Objective: To evaluate the cell-mediated immune response to Toxoplasma gondii (T. gondii) among HIV-infected patients. Methods: Forty HIV-infected patients were studied, Of them, 35 had antibodies to T. gondii and 5 had not, Nine of 35 patients with detectable serum Toxoplasma antibodies were classified as group A1 (CDC 1993), 10 as group B2, 6 as group B3, and 10 as group C3. Peripheral blood mononuclear cells (PBMCs) were obtained by Ficoll-Hypaque gradient centrifugation, Living Toxoplasma gondii trophozoites, herpes simplex virus (HSV), tetanus toroid, and phytohemoagglutinin (PHA) were used in standard proliferation assays, Toxoplasma-responding blasts were expanded and assayed for antigen specificity and HLA restriction by proliferation assays, T cell subsets were analyzed using two-color flow cytometry. Results: Among patients with detectable Toxoplasma serum antibodies, significant PBMC proliferation in response to T. gondii trophozoites was observed in those classified in group Al or B2 but not in those in groups B3 and C3. Toxoplasma-induced blasts from five of six patients after 7 days of culture and from five patients after 15 days of culture proliferated in response to T. gondii in the presence of either autologous or allogeneic PBMCs as antigen-presenting cells (APCs) and/or also proliferated in response to HSV, The surface markers of T. gondii-induced blasts showed a variable percentage of CD4 and CD8 activated cells. Conclusions: T cell proliferative response to living trophozoites of T. gondii is lost only in patients with severe depletion of CD4 cells, PBMC proliferation was observed only in patients with previous T. gondii infection, but the T cell blasts generated showed a strong alloreactivity (proliferating in response to allogeneic irradiated PBMCs) and were apparently not antigen specific (proliferating also in response to HSV).
引用
收藏
页码:741 / 746
页数:6
相关论文
共 12 条
[1]  
[Anonymous], 1992, MMWR Recomm Rep, V41, P1
[2]  
CANESSA A, 1988, J IMMUNOL, V140, P3580
[3]  
COOPER PD, 1967, METHOD VIROL, P243
[4]  
DEROUIN F, 1989, J CLIN LAB IMMUNOL, V28, P179
[5]  
ISRAELSKI DM, 1990, MED MANAGEMENT AIDS, P241
[6]   INVITRO CULTIVATION OF TOXOPLASMA-GONDII CYSTS IN ASTROCYTES IN THE PRESENCE OF GAMMA-INTERFERON [J].
JONES, TC ;
BIENZ, KA ;
ERB, P .
INFECTION AND IMMUNITY, 1986, 51 (01) :147-156
[7]   QUALITATIVE-ANALYSIS OF IMMUNE FUNCTION IN PATIENTS WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROME - EVIDENCE FOR A SELECTIVE DEFECT IN SOLUBLE-ANTIGEN RECOGNITION [J].
LANE, HC ;
DEPPER, JM ;
GREENE, WC ;
WHALEN, G ;
WALDMANN, TA ;
FAUCI, AS .
NEW ENGLAND JOURNAL OF MEDICINE, 1985, 313 (02) :79-84
[8]   TOXOPLASMIC ENCEPHALITIS [J].
LUFT, BJ ;
REMINGTON, JS .
JOURNAL OF INFECTIOUS DISEASES, 1988, 157 (01) :1-6
[9]   IMPAIRED PRODUCTION OF LYMPHOKINES AND IMMUNE (GAMMA) INTERFERON IN THE ACQUIRED IMMUNODEFICIENCY SYNDROME [J].
MURRAY, HW ;
RUBIN, BY ;
MASUR, H ;
ROBERTS, RB .
NEW ENGLAND JOURNAL OF MEDICINE, 1984, 310 (14) :883-889
[10]   TOXOPLASMOSIS OF THE CENTRAL-NERVOUS-SYSTEM IN THE ACQUIRED-IMMUNODEFICIENCY-SYNDROME [J].
PORTER, SB ;
SANDE, MA .
NEW ENGLAND JOURNAL OF MEDICINE, 1992, 327 (23) :1643-1648