SIMULTANEOUS DETERMINATION OF FOLLICLE-STIMULATING-HORMONE AND LUTEINIZING-HORMONE USING A MULTIANALYTE IMMUNOSENSOR

被引：54

作者：

PRITCHARD, DJ ^{[1
]}

MORGAN, H ^{[1
]}

COOPER, JM ^{[1
]}

机构：

[1] UNIV GLASGOW,DEPT ELECTR & ELECT ENGN,GLASGOW G12 8QQ,LANARK,SCOTLAND

来源：

ANALYTICA CHIMICA ACTA | 1995年 / 310卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

BIOSENSORS; MULTIANALYTE; PROTEIN PATTERNING; ANTIBODIES; HORMONES;

D O I：

10.1016/0003-2670(95)00113-E

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A technique is described for constructing a multianalyte immunosensor for the quantitative determination of the human gonadotrophin hormones follicle stimulating hormone (FSH) and luteinising hormone (LH). The assay is a based upon the electrochemical detection of two horseradish peroxidase labelled antibodies using a ferrocene mediated system. The detection limits (3 sigma) were 2.1 U l(-1) FSH and 1.8 U l(-1) LH. There was negligible cross reactivity. Results obtained with the biosensor showed a good correlation with those obtained using an established clinical diagnostic technique based upon dissociation-enhanced lanthanide fluorometric immunoassay. This is the first report of an electrochemical multianalyte immunosensor for high molecular weight analytes.

引用

页码：251 / 256

页数：6

共 19 条

[1] FABRICATION OF SURFACES RESISTANT TO PROTEIN ADSORPTION AND APPLICATION TO 2-DIMENSIONAL PROTEIN PATTERNING [J].

BHATIA, SK ;

TEIXEIRA, JL ;

ANDERSON, M ;

SHRIVERLAKE, LC ;

CALVERT, JM ;

GEORGER, JH ;

HICKMAN, JJ ;

DULCEY, CS ;

SCHOEN, PE ;

LIGLER, FS .

ANALYTICAL BIOCHEMISTRY, 1993, 208 (01) :197-205

[2] MICROPATTERNING PROTEINS AND SYNTHETIC PEPTIDES ON SOLID SUPPORTS - A NOVEL APPLICATION FOR MICROELECTRONICS FABRICATION TECHNOLOGY [J].

BRITLAND, S ;

PEREZARNAUD, E ;

CLARK, P ;

MCGINN, B ;

CONNOLLY, P ;

MOORES, G .

BIOTECHNOLOGY PROGRESS, 1992, 8 (02) :155-160

[3]

BUECHLER KF, 1992, CLIN CHEM, V38, P1678

[4] BIOELECTRONIC INTERFACING - MICRO-FABRICATION AND NANOFABRICATION TECHNIQUES FOR GENERATING PREDETERMINED MOLECULAR ARRAYS [J].

CONNOLLY, P .

TRENDS IN BIOTECHNOLOGY, 1994, 12 (04) :123-127

[5] A METHOD FOR ESTIMATION OF HYDROGEN-PEROXIDE BASED ON MEDIATED ELECTRON-TRANSFER REACTIONS OF PEROXIDASES AT ELECTRODES [J].

FREW, JE ;

HARMER, MA ;

HILL, HAO ;

LIBOR, SI .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1986, 201 (01) :1-10

[6] ELECTROCHEMICAL BIOSENSORS [J].

FREW, JE ;

HILL, HAO .

ANALYTICAL CHEMISTRY, 1987, 59 (15) :A933-&

[7]

GOWENLOCK AH, 1988, VARLEYS PRACTICAL BI

[8]

Green N M, 1975, Adv Protein Chem, V29, P85, DOI 10.1016/S0065-3233(08)60411-8

[9]

Hall EAH, 1990, BIOSENSORS

[10] EXTENDING THE DETECTION LIMIT OF SOLID-PHASE ELECTROCHEMICAL ENZYME-IMMUNOASSAY TO THE ATTOMOLE LEVEL [J].

JENKINS, SH ;

HEINEMAN, WR ;

HALSALL, HB .

ANALYTICAL BIOCHEMISTRY, 1988, 168 (02) :292-299

← 1 2 →