FLOW-INJECTION SYSTEM FOR THE FLUOROMETRIC-DETERMINATION OF FRUCTOSE WITH AN IMMOBILIZED MANNITOL DEHYDROGENASE REACTOR

被引：18

作者：

KIBA, N

INOUE, Y

FURUSAWA, M

机构：

[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 243卷 / 02期

关键词：

FLUOROMETRY; FLOW SYSTEM; FRUCTOSE; ENZYME REACTOR;

D O I：

10.1016/S0003-2670(00)82558-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Immobilized mannitol dehydrogenase is used for the determination of D-fructose in a flow-injection system. The enzyme is immobilized on poly(vinyl alcohol) beads. The oxidation of NADH occurs simultaneously and the disappearance of NADH is measured fluorimetrically. The response is linearly related to fructose concentration in the range 6-600-mu-M; 30 samples per hour can be analysed. The immobilized enzyme retains over 80% of its initial activity after repetitive use for 2 months.

引用

页码：183 / 186

页数：4

共 6 条

[1]

Beutler H. O., 1984, METHOD ENZYMAT AN, VVI, P321

[2] RAPID, AUTOMATED-ANALYSIS OF MONOSACCHARIDES BY HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY OF BORATE COMPLEXES WITH FLUORIMETRIC DETECTION USING 2-CYANOACETAMIDE [J].