FRAGMENTATION STUDIES OF PEPTIDES - THE FORMATION OF Y-IONS

被引：78

作者：

KENNY, PTM

NOMOTO, K

ORLANDO, R

机构：

[1] Suntory Institute of Bioorganic Research, Mishima-Gun, Osaka, 618, Wakayamadai Shimamoto-cho

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 02期

关键词：

D O I：

10.1002/rcm.1290060205

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanism for the formation of y ions in the collision-induced dissociation (CID) spectra of protonated peptides produced by fast-atom bombardment was investigated by tandem mass spectrometry and deuterium labelling studies. The results show that a hydrogen atom attached to nitrogen and not to carbon migrates during cleavage of the amide bond. A mechanism based on these results is presented.

引用

收藏

页码：95 / 97

页数：3

相关论文

共 10 条

[1] CHARACTERIZATION BY TANDEM MASS-SPECTROMETRY OF STRUCTURAL MODIFICATIONS IN PROTEINS [J].

BIEMANN, K ;

SCOBLE, HA .

SCIENCE, 1987, 237 (4818) :992-998

[2] CONTRIBUTIONS OF MASS-SPECTROMETRY TO PEPTIDE AND PROTEIN-STRUCTURE [J].

BIEMANN, K .

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1988, 16 (1-12) :99-111

[3] PROTEIN SEQUENCING BY TANDEM MASS-SPECTROMETRY [J].

HUNT, DF ;

YATES, JR ;

SHABANOWITZ, J ;

WINSTON, S ;

HAUER, CR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (17) :6233-6237

[4] NOVEL FRAGMENTATION PROCESS OF PEPTIDES BY COLLISION-INDUCED DECOMPOSITION IN A TANDEM MASS-SPECTROMETER - DIFFERENTIATION OF LEUCINE AND ISOLEUCINE [J].

JOHNSON, RS ;

MARTIN, SA ;

BIEMANN, K ;

STULTS, JT ;

WATSON, JT .

ANALYTICAL CHEMISTRY, 1987, 59 (21) :2621-2625

[5] COLLISION-INDUCED FRAGMENTATION OF (M+H)+IONS OF PEPTIDES - SIDE-CHAIN SPECIFIC SEQUENCE IONS [J].

JOHNSON, RS ;

MARTIN, SA ;

BIEMANN, K .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1988, 86 :137-154

[6] A COMPARISON OF KEV ATOM BOMBARDMENT MASS-SPECTRA OF PEPTIDES OBTAINED WITH A 2-SECTOR MASS-SPECTROMETER WITH THOSE FROM A 4-SECTOR TANDEM MASS-SPECTROMETER [J].

MARTIN, SA ;

BIEMANN, K .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1987, 78 :213-228

[7] FAST ATOM BOMBARDMENT - A NEW MASS-SPECTROMETRIC METHOD FOR PEPTIDE SEQUENCE-ANALYSIS [J].

MORRIS, HR ;

PANICO, M ;

BARBER, M ;

BORDOLI, RS ;

SEDGWICK, RD ;

TYLER, A .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1981, 101 (02) :623-631

[8] HYDROGEN TRANSFER-REACTIONS IN THE FORMATION OF Y+2 SEQUENCE IONS FROM PROTONATED PEPTIDES [J].

MUELLER, DR ;

ECKERSLEY, M ;

RICHTER, WJ .

ORGANIC MASS SPECTROMETRY, 1988, 23 (03) :217-222

[9] PROPOSAL FOR A COMMON NOMENCLATURE FOR SEQUENCE IONS IN MASS-SPECTRA OF PEPTIDES [J].

ROEPSTORFF, P ;

FOHLMAN, J .

BIOMEDICAL MASS SPECTROMETRY, 1984, 11 (11) :601-601

[10] FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY - A NEW TECHNIQUE FOR THE DETERMINATION OF MOLECULAR-WEIGHTS AND AMINO-ACID-SEQUENCES OF PEPTIDES [J].

WILLIAMS, DH ;

BRADLEY, CV ;

SANTIKARN, S ;

BOJESEN, G .

BIOCHEMICAL JOURNAL, 1982, 201 (01) :105-117