INTERDEPENDENT INFLUENCE OF FOLLICULAR-FLUID ESTRADIOL CONCENTRATION AND MOTILITY CHARACTERISTICS OF SPERMATOZOA ON INVITRO FERTILIZATION RESULTS

被引：6

作者：

DEGEYTER, C ^{[1
]}

DEGEYTER, M ^{[1
]}

SCHNEIDER, HPG ^{[1
]}

NIESCHLAG, E ^{[1
]}

机构：

[1] UNIV MUNSTER,INST REPROD MED,STEINFURTER STR 107,W-4400 MUNSTER,GERMANY

来源：

HUMAN REPRODUCTION | 1992年 / 7卷 / 05期

关键词：

COMPUTERIZED ANALYSIS OF SPERM MOTION; FOLLICULAR FLUID ESTRADIOL; MOVEMENT PARAMETERS OF SPERMATOZOA; OOCYTE MATURITY; SPERM CAPACITATION;

D O I：

10.1093/oxfordjournals.humrep.a137716

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Methods are presented for an objective assessment of the quality of both gametes in an in-vitro fertilization programme. The concentrations of oestradiol and progesterone in follicles whose oocytes did or did not fertilize, were measured and assessed as potential markers of oocyte maturity. There was no difference in the mean concentrations of either steroid in follicular fluid of fertilized and unfertilized oocytes. However, a highly significant inverse correlation was observed between the oestradiol concentration in follicles of oocytes becoming fertilized and the number of spermatozoa added for fertilization (P < 0.001). Follicular fluid concentrations of progesterone did not correlate with the number of inseminated spermatozoa. The correlation between follicular oestradiol concentrations and the number of spermatozoa needed for fertilization was then used to identify movement characteristics of spermatozoa in the culture medium which were relevant for fertilization. Sufficient numbers of spermatozoa having specific values of head cross frequency, lateral head displacement, linearity and curvilinear velocity were critical for the occurrence of fertilization in vitro.

引用

页码：665 / 670

页数：6

共 23 条

[1]

Ben-Rafael Z., Meloni F., Strauss J.F., Hi Blasco K., Mastroianni L., Flickinger G.L., Relationships between polypronuclear fertilization and follicular fluid hormones in gonadotropin-treated women, Fertil. Steril, 47, pp. 284-288, (1987)

[2]

Botero-Ruiz W., Laufer N., Dechemey A.H., Polan M.L., Haseltine F.P., Behrman H.R., The relationship between follicular fluid steroid concentration and successful fertilization of human oocytes in vitro. Fertil, Steril, 41, pp. 820-826, (1984)

[3]

Burkman L.J., Coddington C.C., Franken D.R., Kruger T.F., Rosen Waks Z., Hodgen G.D., The hcmizona assay (HZA): Development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil, Steril, 49, pp. 688-697, (1988)

[4]

Carson R.S., Trounson A.O., Findlay J.K., Successful fertilization of human oocytes in vitro: Concentration of estradiol-17-beta, progesterone and androstenedione in the antral fluid of donor follicles, J. Clin. Endocrinol. Metab, 55, pp. 798-800, (1982)

[5]

Chan S.Y.W., Wang C., Chan S.T.H., Ho P.C., Differential evaluation of human sperm hypoosmotic swelling test and its relationship with the outcome of in-vitro fertilization of human oocytes. Hum, Reprod, 5, pp. 84-88, (1990)

[6]

De Geyter C.H., Bals-Pratsch M., Doeren M., Yeung C.-H., Grunert J.H., Bordt J., Schneider H.P.G., Nieschlag E., Human and bovine cervical mucus penetration as a test of sperm function for in-vitro fertilization. Hum, Reprod, 3, pp. 948-954, (1988)

[7]

Franchimont P., Hazee-Hagelstein M.-T., Hazout A., Frydman R., Schatz B., Demerle F., Correlation between follicular fluid content and the results of in vitro fertilization and embryo transfer. I. Sex steroids. Fertil, Steril, 52, pp. 1006-1011, (1989)

[8]

Grunert J.-H., De Geyter C.H., Bordt J., Schneider H.P.G., Nieschlag E., Does computerized image analysis of sperm movement enhance the predictive value of semen analysis for in-vitro fertilization results, Int. J. Androl, 12, pp. 329-338, (1989)

[9]

Kxeiner D., Liu H.-C., Itskovitz J., Veeck L.L., Rosenwaks Z., Follicular fluid estradiol and progesterone are markers of preovulatory oocyte quality. Fertil, Steril, 48, pp. 991-994, (1987)

[10]

Kruger T.F., Menkveld R., Stander F.S.H., Lombard C.J., Van Der Merwe J.P., Van Zyl J.A., Smith K., Sperm morphologic features as a prognostic factor in in vitro fertilization. Fertil, Steril, 46, pp. 1118-1123, (1987)

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