A NEW CELL ASSAY TO DETERMINE THE VIRULENCE OF FRANCISELLA-TULARENSIS

被引：6

作者：

MACK, K

FULOP, M

MANCHEE, RJ

STIRLING, CJ

机构：

[1] CHEM & BIOL DEF ESTAB,SALISBURY,WILTS,ENGLAND

[2] UNIV MANCHESTER,SCH BIOL SCI,MANCHESTER,LANCS,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 19卷 / 03期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00931.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cell culture assay to determine the virulence of Francisella tularensis was devised. Murine cell lines P388 and J774 were significantly more susceptible to F. tularensis Schu4 than the attenuated live vaccine strain. The ability of F. tularensis strains to cause cell death correlated with their virulence to mice. Use of this assay with infected cells separated from susceptible uninfected cells by a membrane with 0.1 mu m pores, failed to demonstrate possible diffusible exotoxins produced by F. tularensis.

引用

页码：158 / 160

页数：3