ONLINE SOLID-PHASE EXTRACTION OF CEFTAZIDIME IN SERUM AND DETERMINATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：18

作者：

BOMPADRE, S

FERRANTE, L

ALO, FP

LEONE, L

机构：

[1] UNIV ANCONA,FAC MED,INST BIOMED SCI,I-60100 ANCONA,ITALY

[2] UNIV ANCONA,FAC MED,CHAIR VASC SURG,I-60100 ANCONA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 669卷 / 02期

关键词：

D O I：

10.1016/0378-4347(95)00100-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A column-switching high-performance liquid chromatographic assay is described for the determination of ceftazidime (a third-generation cephalosporin) in human serum. The method does not require prior sample pretreatment. Serum is directly injected in a first chromatographic column for sample clean-up and extraction. Thereafter, using an on-line column-switching system, the drug is quantitatively transferred and separated on a second, analytical column followed by determination using ultraviolet absorption at 258 nm. The technique allows direct, rapid, precise, and simple determination of ceftazidime in serum over the range of 1-250 mu g/ml using 12.5 mu l of serum. This method was applied to study the pharmacokinetics of the drug in patients undergoing vascular surgery.

引用

页码：265 / 269

页数：5

共 8 条

[1] HOSPITAL ROUTINE ANALYSIS OF PENICILLINS, 3RD-GENERATION CEPHALOSPORINS AND AZTREONAM BY CONVENTIONAL AND HIGH-SPEED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].