RETROVIRAL VECTOR SEQUENCES INHIBIT HUMAN BETA-GLOBIN GENE-EXPRESSION IN TRANSGENIC MICE

被引：33

作者：

MCCUNE, SL

TOWNES, TM

机构：

[1] UNIV ALABAMA,SCH MED,DEPT BIOCHEM & MOLEC GENET,BIRMINGHAM,AL 35294

[2] UNIV ALABAMA,SCH DENT,BIRMINGHAM,AL 35294

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 21期

关键词：

D O I：

10.1093/nar/22.21.4477

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The DNase I hypersensitive site 5' HS2 of the human beta-globin locus control region confers position-independent, high-level expression on the human beta-globin gene in transgenic mice. When a 5' HS2 beta-globin construct is flanked by retroviral vector sequences derived from Moloney Murine Leukemia Virus (MoMLV), expression of the beta-globin gene is severely inhibited. Apparently, one or more elements within the MoMLV genome is capable of repressing transcription of the human beta-globin gene in transgenic mice. A construct lacking the retroviral enhancer also fails to express the beta-globin gene, indicating that this region of the virus is not essential for repression. Further analysis may permit the identification of specific viral sequences that inhibit gene expression; these sequences could then be deleted or mutated to produce improved viral vectors.

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页码：4477 / 4481

页数：5

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