FIBRONECTIN BINDING-PROPERTIES OF BACTERIOLOGIC PETRI PLATES AND TISSUE-CULTURE DISHES

Serum proteins are more effective in inhibiting fibronectin binding to bacteriologic petri plates than to tissue culture dishes. The fibronectin binding properties of plastic substrata could be enhanced by oxidation with H//2SO//4 or diminished by dissolution and recasting of tissue culture dishes. Plastics with enhanced fibronectin binding properties (tissue culture plates) were found to be hydrophilic and good substrates for cell attachment and growth while plastics with decreased fibronectin binding characteristics were found to be hydrophobic and poor substrates for cell attachment and growth. The cell-adhesive properties of bacteriologic and tissue culture plastic substrata were found to vary during incubation with cells. While cells remained firmly attached and spread on tissue culture plastics over a period of 5 days or more, previously attached cells gradually detached from bacteriologic plastics at incubation times beyond 12 h.