DETERMINATION OF 3-HYDROXYKYNURENINE IN HUMAN BRAIN AND PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION - INCREASED CONCENTRATIONS IN HEPATIC-ENCEPHALOPATHY

被引：53

作者：

PEARSON, SJ ^{[1
]}

REYNOLDS, GP ^{[1
]}

机构：

[1] UNIV SHEFFIELD,DEPT BIOMED SCI,SHEFFIELD S10 2TN,S YORKSHIRE,ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 565卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80406-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and specific method was developed for the determination of 3-hydroxykynurenine in brain tissue and blood plasma using high-performance liquid chromatography with electrochemical detection. This involved an extraction procedure using strong cation-exchange columns and also permitted the determination of 3-hydroxyanthranilic acid in brain tissue. The method was applied to the investigation of post mortem brain tissue from patients with hepatic encephalopathy. Cortical 3-hydroxykynurenine concentrations were substantially increased in such patients above control values, providing evidence for a dysfunction of tryptophan metabolism in this disease.

引用

页码：436 / 440

页数：5

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