EFFECT OF ESTROGEN STATUS ON SERUM LEVELS OF GROWTH HORMONE-BINDING PROTEIN AND INSULIN-LIKE GROWTH FACTOR-I IN NONPREGNANT AND PREGNANT-WOMEN

OBJECTIVE Since there appears to be a relationship between circulating oestrogens and growth hormone, we have investigated the effect of the oestrogen status of adult women on serum levels of GHBP and IGF-I. DESIGN AND PATIENTS The investigation was performed on serum samples of 14 spontaneously menstruating women, 10 women taking oral contraceptives containing 20-50 mug ethinyloestradiol, and 30 pregnant women at different stages of pregnancy. MEASUREMENTS Serum levels of GHBP were measured by HPLC gel filtration and IGF-I levels were measured by RIA after acid-ethanol extraction. RESULTS In the spontaneously menstruating women the mean +/- SD serum level of GHBP was 34.6 +/- 6-7% and of IGF-I 30 +/- 7 nmol/l. Serum GHBP levels were negatively (r = - 0.67; P < 0.01) and IGF-I levels were positively related (r = 0.69; P < 0.01) to serum oestradiol concentrations. In the women taking oral contraceptives serum levels of GHBP were 47.0 +/- 7.4%. This was significantly (P < 0.001) higher than in spontaneously menstruating women. In contrast, IGF-I levels were not different from those obtained in spontaneously menstruating women. In the pregnant women, the mean +/- SD serum level of GHBP was not different from that observed in non-pregnant spontaneously menstruating women. Polynomial regression analysis, however, showed a significant (P = 0.01) second-order relationship between the duration of pregnancy and serum GHBP levels, with increasing levels during the first half of pregnancy and decreasing levels thereafter. Serum concentrations of IGF-I increased during the second half of pregnancy and were significantly (P < 0.005) elevated in the third trimester. CONCLUSIONS In non-pregnant women the endogenous oestrogen status seems to modulate negatively GHBP levels and positively IGF-I levels, whereas oral oestrogen administration, in contrast, increases serum levels of GHBP without modification of IGF-I levels. During pregnancy serum GHBP levels increase slightly during the first half of pregnancy and decrease thereafter, whereas IGF-I concentrations increase during the second part of pregnancy. The oestrogen status of women has a complex effect on serum concentrations of GHBP and IGF-I and has therefore to be taken into account when evaluating serum levels of GHBP and IGF-I.