INDUCTION AND DEVELOPMENT OF POTATO-TUBERS IN A JAR FERMENTER

被引:27
作者
AKITA, M [1 ]
TAKAYAMA, S [1 ]
机构
[1] KYOWA HAKKO KOGYO CO LTD, TSUKUBA RES LABS, TSUKUBA, IBARAKI 305, JAPAN
关键词
CULTURE TEMPERATURE; IN-VITRO TUBERIZATION; MICROTUBER; SOLANUM-TUBEROSUM L; TIME COURSE;
D O I
10.1007/BF00037717
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Potato (Solanum tuberosum L.) tubers were mass propagated in a jar fermentor using a two-step method consisting of a shoot multiplication step (phase 1) followed by a tuber induction and development step (phase 2). Tuberization was observed within 2 weeks of phase 2 and the number of tubers did not increase after this culture period. In contrast, total tuber weight continuously increased for at least 10 weeks. Although the number of tubers and the total tuber weight clearly decreased under the lower temperature (17-degrees-C), this weight decrease was partially prevented by changing the temperature from 17-degrees-C to 25-degrees-C after 2 weeks of phase 2. This result indicates that tuber development can be controlled independently of induction. Lower temperatures influenced the localization and size distribution of tubers in the jar fermentor.
引用
收藏
页码:177 / 182
页数:6
相关论文
共 12 条
[1]  
AKITA M, 1988, Acta Horticulturae (Wageningen), P55
[2]  
AKITA M, 1988, 1988 JAP SOC HORT SC, P266
[3]  
AKITA M, 1987, 1987 JAP SOC HORT SC, P266
[4]  
CHANDRA R, 1988, INT ASS PLANT TISSUE, V55, P10
[5]   INDUCTION OF INVITRO TUBERS IN A BROAD RANGE OF POTATO GENOTYPES [J].
ESTRADA, R ;
TOVAR, P ;
DODDS, JH .
PLANT CELL TISSUE AND ORGAN CULTURE, 1986, 7 (01) :3-10
[6]   FACTORS AFFECTING THE FORMATION OF INVITRO TUBERS OF POTATO (SOLANUM-TUBEROSUM-L) [J].
HUSSEY, G ;
STACEY, NJ .
ANNALS OF BOTANY, 1984, 53 (04) :565-578
[7]  
LEVIN R, 1989, IAPTC NEWSLETTER, V59, P2
[8]  
MILLER SA, 1984, HDB PLANT CELL CULTU, V3, P291
[9]  
MULLERROBER BT, 1990, MOL GEN GENET, V224, P136, DOI 10.1007/BF00259460
[10]   A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES [J].
MURASHIGE, T ;
SKOOG, F .
PHYSIOLOGIA PLANTARUM, 1962, 15 (03) :473-497