HSP70 BINDS SPECIFICALLY TO A PEPTIDE DERIVED FROM THE HIGHLY CONSERVED DOMAIN (I) REGION OF P53

被引:39
作者
LAM, KT
CALDERWOOD, SK
机构
[1] HARVARD UNIV, SCH MED, DANA FARBER CANC INST, BOSTON, MA 02115 USA
[2] HARVARD UNIV, SCH MED, JOINT CTR RADIAT THERAPY, BOSTON, MA 02115 USA
关键词
D O I
10.1016/0006-291X(92)91174-O
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Products of a number of mutant p53 genes bind with high affinity to members of the hsp70 family of chaperonin proteins, whereas wild type p53 lacks this type of association. Examination of the sequences of p53 genes from five different species enabled us to predict domains on p53 which may be involved in the association with hsp70 family members. A synthetic polypeptide (Pro-17-Gly) corresponding to the candidate hsp70 binding domain bound to in vitro translated hsp70 as determined by affinity chromatography and nondenaturing gel mobility shift assays. In addition, the Pro-17-Gly peptide competitively inhibited association between hsp70 and p53, an activity which was determined by immunoprecipitation with anti-p53 monoclonal antibody PAb240. The data indicate that p53 contains a hsp70 binding domain, which is located in a highly conserved region at the amino terminus of the protein, and may participate in the cellular function of wild-type p53 or in the transforming capacity of p53 mutants. © 1992.
引用
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页码:167 / 174
页数:8
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