PURIFICATION AND CHARACTERIZATION OF CYTOSOLIC 6-PHOSPHOGLUCONATE DEHYDROGENASE ISOZYMES FROM MAIZE

被引：24

作者：

BAILEYSERRES, J

NGUYEN, MT

机构：

[1] Dept. of Botany and Plant Sciences, University of California, Riverside

来源：

PLANT PHYSIOLOGY | 1992年 / 100卷 / 03期

关键词：

D O I：

10.1104/pp.100.3.1580

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cytosolic isozymes of 6-phosphogluconate dehydrogenase were purified from roots of maize (Zea mays L.). The final preparation contained two 55-kD proteins. Affinity-purified dehydrogenases from a maize line that is null for both cytosolic 6-phosphogluconate dehydrogenase isozymes (Pgd1-null, Pgd2-null) lacked the 55-kD proteins. The substrate kinetics of the purified enzyme were determined.

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页码：1580 / 1583

页数：4

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