IDENTIFICATION OF AMINO-ACID BASE CONTACTS IN THE MYC DNA COMPLEX BY SITE-SPECIFIC BROMOURACIL MEDIATED PHOTO-CROSS-LINKING

被引：23

作者：

DONG, QP

BLATTER, EE

EBRIGHT, YW

BISTER, K

EBRIGHT, RH

机构：

[1] RUTGERS STATE UNIV,DEPT CHEM,NEW BRUNSWICK,NJ 08855

[2] RUTGERS STATE UNIV,WAKSMAN INST,NEW BRUNSWICK,NJ 08855

[3] UNIV COLOGNE,SCH MED,INST BIOCHEM,D-50924 COLOGNE,GERMANY

来源：

EMBO JOURNAL | 1994年 / 13卷 / 01期

关键词：

AMINO ACID-BASE CONTACTS; DNA BINDING; MYC; PHOTO-CROSS-LINKING; PROTEIN-DNA INTERACTION;

D O I：

10.1002/j.1460-2075.1994.tb06249.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Myc binds to a 6 bp 2-fold symmetric DNA site: 5'-C(-3)A(-2)C(-1)G(+1)T(+2)G(+3)-3'. Using site-specific 5-bromouracil mediated photocrosslinking, we show that His336 of Myc contacts, or is close to, the thymine 5-methyl group at 2-fold symmetry-related positions -2 and +2 of the DNA site in the Myc- DNA complex. Our results strongly suggest that homologous amino acids of Myc and Max make equivalent contacts in the respective protein-DNA complexes.

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收藏

页码：200 / 204

页数：5

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