IDENTIFICATION OF AMINO-ACID BASE CONTACTS IN THE MYC DNA COMPLEX BY SITE-SPECIFIC BROMOURACIL MEDIATED PHOTO-CROSS-LINKING

被引:23
作者
DONG, QP
BLATTER, EE
EBRIGHT, YW
BISTER, K
EBRIGHT, RH
机构
[1] RUTGERS STATE UNIV,DEPT CHEM,NEW BRUNSWICK,NJ 08855
[2] RUTGERS STATE UNIV,WAKSMAN INST,NEW BRUNSWICK,NJ 08855
[3] UNIV COLOGNE,SCH MED,INST BIOCHEM,D-50924 COLOGNE,GERMANY
关键词
AMINO ACID-BASE CONTACTS; DNA BINDING; MYC; PHOTO-CROSS-LINKING; PROTEIN-DNA INTERACTION;
D O I
10.1002/j.1460-2075.1994.tb06249.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Myc binds to a 6 bp 2-fold symmetric DNA site: 5'-C(-3)A(-2)C(-1)G(+1)T(+2)G(+3)-3'. Using site-specific 5-bromouracil mediated photocrosslinking, we show that His336 of Myc contacts, or is close to, the thymine 5-methyl group at 2-fold symmetry-related positions -2 and +2 of the DNA site in the Myc- DNA complex. Our results strongly suggest that homologous amino acids of Myc and Max make equivalent contacts in the respective protein-DNA complexes.
引用
收藏
页码:200 / 204
页数:5
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